SMU Medical Journal

Ganeva Silviya ¹, Todorova Katya ¹, Lukanov Tsvetan ², Rayanova Ginka ¹, Blajeva Svetla ², Tsvyatkovska Tsvetomira ¹

Affiliations
1 Medical University Pleven, UMHAT “D-r G. Stranski”, Clinic of Endocrinology and Metabolic Diseases, BG
2 Medical University Pleven, UMHAT “D-r G. Stranski”, Medico-Diagnostic Laboratory of Immunology, BG

Abstract

A considerable suggestion exists between the leukocyte count, lymphocyte (lymph) dysfunction and the possibility of developing DMt2. Aim of the study was to investigate the CD19 +expression of B- lymph in peripheral blood in patients with metabolic syndrome (MS) without and with Diabetes mellitus type 2 (DMt2). A prospective, one-year, comparative, observational study was performed. In 26 MS patients without DMt2 (n₁=26) and 69 with DMt2 (n₂=69) were measured and compared the levels of leucocytes and CD19+ B- lymph subtypes in peripheral blood by flow-cytometric analysis. The leucocytes count is in normal range in both groups with MS. DMt2 patients have a higher leucocytes count (n₁=6.93±1.92.10⁹ vs n₂=7.27±1.85.10⁹; р= 0,04) in the referent range. The percentage of the common lymphocytes population was also higher among n1 patients compared to n2, but without significant difference in their absolute count [(n₁=38,26%±7,43% vs. n₂=33,42%±7,08%) and (n₁=2.58±0.8.10⁹ vs. n₁=3,45±0,67.10⁹)]. The percentage ratio and absolute count of СD19+ B- lymphocytes subtypes in patients from both groups was lower than referent values, but without significant difference between the two groups.The levels of СD19+B-lymphocytes in peripheral blood are lower in patients with MS irrespective of the level of glycaemia.

Keywords

Metabolic Syndrome, B-Lymphocytes, Type 2 Diabetes Mellitus Risk, Flow-Cytometry.

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